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铁道科学与工程学报

JOURNAL OF RAILWAY SCIENCE AND ENGINEERING

Vol. 17    No. 2    April 2007

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Mutagenic breeding of silver-resistant Acidithiobacillus
ferrooxidans and exploration of resistant mechanism
WU Xue-ling(吴学玲), QIU Guan-zhou(邱冠周), GAO Jian(高 健),
DING Jian-nan(丁建南), KANG Jian(康 健), LIU Xin-xing(刘新星)

Key Laboratory of Biometallurgy of Ministry of Education,
School of Resources Processing and Bioengineering, Central South University, Changsha 410083, China

Abstract:The silver-resistant Acidithiobacillus ferrooxidans were isolated from 22 acid mine drainage (AMD) samples collected from Dexing Copper Mine and Chengmen Mountain Mine, Jiangxi Province, China. Isolate DX16 is obtained from the sample taken from Dexing Copper Mine and still carries out ferrous ion oxidation when incubated in 9K medium containing silver nitrate (240 mg/L). While isolate H1, a less resistant strain taken from Yin Mountain Mine, has a tolerate level of only 60 mg/L. Based on 16S ribosomal DNA sequencing, both bacterial 16SrDNA sequences are 100% similar to Acidithiobacillus ferrooxidans ATCC 23270. Through ultraviolet irradiation induced mutations, isolate mDX16 that is obtained from DX16 carries out ferrous ion oxidation when incubated in 9K medium containing higher concentration of silver nitrate (250 mg/L). When silver-resistant gene (SilC) analysis is carried out on the two isolates, it is seen that this gene was absent in both.

 

Key words: silver resistance; isolation and identification; UV mutation; Acidithiobacillus ferrooxidans; resistance mechanism

ISSN 1672-7029
CN 43-1423/U

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