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铁道科学与工程学报

JOURNAL OF RAILWAY SCIENCE AND ENGINEERING

Vol. 18    No. 6    December 2008

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Growth and surface properties of new thermoacidophilic Archaea strain Acidianus manzaensis YN-25 grown on different substrates
HE Huan(何 环), YANG Yi(杨 益), XIA Jin-lan(夏金兰),
DING Jian-nan(丁建南), ZHAO Xiao-juan(赵小娟), NIE Zhen-yuan(聂珍媛)

Key Laboratory of Biometallurgy of Ministry of Education,
School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China

Abstract:The growth and surface properties of new thermoacidophilic Archaea strain Acidianus manzaensis YN-25 isolated from an acid hot spring in Tengchong, Yunnan Province, China were investigated cultured on different substrates including soluble substrate ferrous sulfate and nonsoluble solid substrates S0, pyrite and chalcopyrite. The growth characteristics of the cells in each substrate were characterized with the changes in cell number, pH, Eh, and concentrations of Fe2+ or SO42−, or ratios of [Fe2+] to [Fe3+], and the surface properties were characterized and analyzed in terms of Zeta-potential, hydrophobicity, and surface FT-IR spectra of the cells. The results show that the cells grown on solid substrates have higher value of isoelectric points. They are more hydrophobic and express more surface proteins than ferrous sulfate grown cells.

 

Key words: surface chemistry; Acidianus manzaensis; bioleaching; extracellular polymers

ISSN 1672-7029
CN 43-1423/U

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