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铁道科学与工程学报

JOURNAL OF RAILWAY SCIENCE AND ENGINEERING

Vol. 18    No. 6    December 2008

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Liquid-nitrogen cryopreservation of three kinds of
autotrophicbioleaching bacteria
WU Xue-ling(吴学玲)1, XIN Xiao-hong(辛晓红)2, JIANG Ying(蒋 莹)1,
LIANG Ren-xing(梁任星)1, YUAN Peng(袁 鹏)1, FANG Cheng-xiang(方呈祥)

1. Key Laboratory of Biometallurgy of Ministry of Education,
School of Minerals Processing and Bioengineering, Central South University,
Changsha 410083, China;
2. China Center for Type Culture Collection, College of Life Science, Wuhan University,
Wuhan 430072, China

Abstract:Three kinds of autotrophic bioleaching bacteria strains, including mesophilic and acidophilic ferrous ion-oxidizing bacteria Acidithiobacillus ferrooxidans (A. ferrooxidans), mesophilic and acidophilic sulfur-oxidizing bacteria Acidithiobacillus thiooxidans (A. thiooxidans), and moderately thermophilic sulfur-oxidizing bacteria Acidianus brierleyi, were cryopreserved in liquid nitrogen and their ferrous ion- or sulfur-oxidizing activities were investigated and compared with the original ones. The results revealed that ferrous ion/sulfur oxidation activities of the strains were almost equal before and after cryopreservation. Glycerin was used as cryoprotective agent. In conclusion, liquid-nitrogen cryopreservation is a simple and effective method for autotrophic bioleaching microorganisms.

 

Key words: A. ferrooxidans; A. thiooxidans; Acidianus brierleyi; cryopreservation; liquid nitrogen; growth activities

ISSN 1672-7029
CN 43-1423/U

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