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铁道科学与工程学报

JOURNAL OF RAILWAY SCIENCE AND ENGINEERING

Vol. 18    No. 6    December 2008

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Repetitive sequence based polymerase chain reaction to
differentiate close bacteria strains in acidic sites
XIE Ming(谢 明)1, 2, YIN Hua-qun(尹华群)1, 2, LIU Yi(刘 毅)1, 2,
LIU Jie(刘 杰)1, 2, LIU Xue-duan(刘学端)1, 2

Key Laboratory of Biometallurgy of Ministry of Education,
School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China

Abstract:To study the diversity of bacteria strains newly isolated from several acid mine drainage(AMD) sites in China, repetitive sequence based polymerase chain reaction (rep-PCR), a well established technology for diversity analysis of closely related bacteria strains, was conducted on 30 strains of bacteria Leptospirillum ferriphilium, 8 strains of bacteria Acidithiobacillus ferrooxidans, as well as the Acidithiobacillus ferrooxidans type strain ATCC (American Type Culture Collection) 23270. The results showed that, using ERIC and BOX primer sets, rep-PCR produced highly discriminatory banding patterns. Phylogenetic analysis based on ERIC-PCR banding types was made and the results indicated that rep-PCR could be used as a rapid and highly discriminatory screening technique in studying bacterial diversity, especially in differentiating bacteria within one species in AMD.

 

Key words: rep-PCR; diversity; acid mine drainage; Acidithiobacillus ferrooxidans; Leptospirillum ferriphilium

ISSN 1672-7029
CN 43-1423/U

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