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铁道科学与工程学报

JOURNAL OF RAILWAY SCIENCE AND ENGINEERING

Vol. 19    No. 3    June 2009

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Characterization of cytochrome mutants for pellicle formation in Shewanella onedensis MR-1
LIANG Yi-li(梁伊丽)1, 2, HE Zhi-li(贺志理)2, GAO Hai-chun(高海春)2,
QIU Guan-zhou(邱冠周)1, ZHOU Ji-zhong(周集中)1, 2, LIU Xue-duan(刘学端)1

1. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;
2. Institute for Environmental Genomics and Department of Botany and Microbiology, University of Oklahoma,
Norman 73019, USA

Abstract:Biofilm systems are effective for biosorption of metal ions. Shewanella oneidensis MR-1, a Gram-negative facultative anaerobe, is a natural pellicle-like biofilm former. The mechanisms of pellicle formation by S. oneidensis MR-1 have not yet been understood. 17 S. oneidensis MR-1 deletion mutants, including 12 c-type cytochromes were generated and tested if they were involved in pellicle formation. The results show that ∆SO4666, ∆SO1777, SO1782, SO2361 and ∆SO2363 have varying deficiency in pellicle formation. The deletion mutant ∆SO4666 cannot form a pellicle under non-shake conditions, suggesting that it may play an important role in pellicle formation by S. oneidensis MR-1. Overall, these data suggest a very complex picture of aerobic respiration by S. oneidensis MR-1.

 

Key words: cytochrome; mutant; electron transport; pellicle; biofilms

ISSN 1672-7029
CN 43-1423/U

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