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铁道科学与工程学报

JOURNAL OF RAILWAY SCIENCE AND ENGINEERING

Vol. 11    No. 5    October 2001

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UV-induced mutagenesis of oxidation activity of
ferrous ion of Thiobacillus ferrooxidans
ZHANG Zai-hai(张在海),  QIU Guan-zhou(邱冠周), HU Yue-hua(胡岳华),
FU Jian-hua(付建华), LIU Xiao-rong(刘晓荣) 

Department of Mineral Engineering, Central South University,
Changsha 410083, P.R.China

Abstract: An excellent strain named T.f6 was isolated and screened, the dose and other condition for the UV-induced mutagenesis were studied and the richened positive mutant m+ T.f6 was applied in the column leaching of copper-containing sulfides. The results show that T.f6 is characterized by rapid oxidation of ferrous ion and cupric sulfide, high tolerance of toxic ion and short generation time. The best mutagenic effectiveness can be obtained under the dose of low kill rate of UV and low temperature treatment, under which the best richened m+ T.f6 can be shortened 1.4h.It was shown by the column leaching of copper that the leaching rate can be enhanced by at least 11% compared with the original one by the mutants.

 

Key words: Thiobacillus ferrooxidans; mutagenesis; bacterial leaching

ISSN 1672-7029
CN 43-1423/U

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